Research leaders utilizing the EPSCoR Plant Transformation Facility are required to submit an application summarizing their research objectives, funding, and personnel who will be assisting with the project. Profiles of current or pending research projects for which applications have been received are presented below.
Project Title: Transformation System for Loblolly Pine
Project Leaders: Yinghua Huang and C.G. Tauer, Dep. of Forestry, O.S.U.
Sponsoring Agency: Oklahoma Agricultural Experiment Station
Objective: Development of tissue culture and transformation methodologies for loblolly pine.
Project Title: EvalTuatin
Project Leaders: Margaret Pierce and Margaret Essenberg, Department of Biochemistry and Molecular Biology, Oklahoma State University
Sponsoring Agency: National Science Foundation, O.A.E.S.
Objective: Test the necessity of phytoalexins for resistance to bacterial blight in cotton by producing transgenic plants with altered ability to accumulate phytoalexins.
Project Title: Development of a Transformation System for Ice Plant
Project Leaders: John Cushman, Dep. of Bioch. & Molec. Biol., O.S.U.
Sponsoring Agency: Oklahoma Agricultural Experiment Station
Objective: Development of tissue culture and transformation methodologies for common ice plant.
Project Title: Molecular Mechanisms of CAM Induction by Salinity Stress
Project Leaders: John Cushman, Dep. of Bioch. and Molec. Biol., O.S.U.
Sponsoring Agency: USDA National Research Initiative
Objective: Dissect cis-acting elements in the 5' flanking regions of salt-inducible genes from the common ice plant.
Project Title: Intercellular Transport of Proteins
Project Leaders: Biao Ding, Department of Botany, Oklahoma State University
Sponsoring Agency: Samuel Robert Noble Foundation
Objective: Determine how proteins move from cell-to-cell by introducing chimeric gene constructs into detached tobacco leaves.
Project Title: In Planta Transformation of Arabidopsis
Project Leaders: John Cushman, Dep. of Bioch. and Molec. Biol., O.S.U.
Sponsoring Agency: Oklahoma Agricultural Experiment Station
Objective: To introduce chimeric gene constructs containing LEA genes (from soybean) into Arabidopsis to evaluate their role in dissection tolerance.
Project Title: Role of Mannitol in Desiccation Tolerance of Wheat
Project Leaders: Arron Guenzi and Bjorn Martin, Dep. of Plant and Soil Sciences, O.S.U.
Sponsoring Agency: Oklahoma Agricultural Experiment Station
Objective: Create transgenic wheat which accumulates mannitol in the cytoplasm or chloroplast and evaluate progeny for drought tolerance.
Project Title: Improving Fungal Resistance in Wheat
Project Leaders: Arron Guenzi, Dep. of Plant and Soil Sciences, O.S.U.
Sponsoring Agency: Oklahoma Agricultural Experiment Station
Objective: Create transgenic wheat which accumulate chitinases and glucanases and evaluate progeny for resistance to fungal diseases.
Project Title: Genetic Manipulation of Flower Development in Sweetgum
Project Leaders: Yinghua Huang and C.G. Tauer, Dep. of Forestry, O.S.U.
Sponsoring Agency: Oklahoma Agricultural Experiment Station
Objective: This project focuses on isolation, characterization, and function analysis of genes useful for engineering reproductive sterility in sweetgum.
Project Title: Coat Protein Mediated Resistance to Wheat Streak Mosaic Virus
Project Leaders: John Sherwood, Dep. of Plant Pathology, O.S.U.
Sponsoring Agency: Oklahoma Agricultural Experiment Station
Objective: Produce transgenic wheat plants which overproduce the coat protein of wheat streak mosaic virus (WSMV) and evaluate whole plant resistance to WSMV.
Project Title: Alteration of Lignin Biosynthesis in Bermudagrass
Project Leaders: Arron Guenzi and Charles Taliaferro, Dep. of Plant and Soil Sciences, O.S.U.
Sponsoring Agency: Oklahoma Agricultural Experiment Station
Objective: Alter lignin biosynthesis in bermudagrass by creating transgenic plants with sense and anti-sense constructs of the caffeic acid methyl transferase gene. Evaluate transgenic material for lignin quantity and quality.
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